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Proceedings Paper

Active sorting switch for biological objects
Author(s): Mojmír Šerý; Zdeněk Pilát; Alexander Jonáš; Jan Ježek; Petr Jákl; Pavel Zemánek; Ota Samek; Ladislav Nedbal; Martin Trtílek
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Paper Abstract

Active contactless optical sorting of microobjects represents very useful technique in many areas of biology, chemistry, and medicine. We suggest here a configuration that combines optical sorting, trapping, excitation, and detection paths and provides efficient sorting of biological samples according to their various parameters (fluorescence, Raman spectrum, CCD image, motion etc.). This approach is based on the shape of the laser beam and we succeeded in sorting of several types of living microorganisms.

Paper Details

Date Published: 28 August 2010
PDF: 7 pages
Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 776210 (28 August 2010); doi: 10.1117/12.859859
Show Author Affiliations
Mojmír Šerý, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Zdeněk Pilát, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Alexander Jonáš, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Ježek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Petr Jákl, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Ladislav Nedbal, Institute of Systems Biology and Ecology of the ASCR, v.v.i. (Czech Republic)
Martin Trtílek, Photon Systems Instruments (Czech Republic)


Published in SPIE Proceedings Vol. 7762:
Optical Trapping and Optical Micromanipulation VII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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