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Proceedings Paper

The microstructure of mandibular bone grafts and three-dimensional cell clusters
Author(s): Sarper Gürel; Corinne Unold; Hans Deyhle; Georg Schulz; Sebastian Kühl; Belma Saldamli; Jutta Tübel; Rainer Burgkart; Felix Beckmann; Bert Müller
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Paper Abstract

During the last decade, several tissues and biomaterials for medical applications in replacing bony tissues have been developed. Three-dimensional cell clusters and mandibular bone grafts are two distinct examples of these developments. The characterization of the complex three-dimensional structures, however, is still mainly restricted on the twodimensional analysis of histological slices. The present paper examines the quantitative analysis of mandibular bone grafts and three-dimensional cell clusters on the basis of synchrotron radiation-based micro computed tomography measurements. An automated search of pre-defined microstructures through component labeling is applied to the real datasets in order to identify features that reside independently from other components. The examples demonstrate three levels of complexity: rather large pieces of bone augmentation material that touch each other, individual adipocytes difficult to automatically segment in a wet cluster and osmium-stained adipocyte exhibiting higher X-ray absorption than the surrounding tissue. Although the structures of interest such as the cells can be labeled, de-clustering of the components requires the incorporation of erosion and dilation algorithms.

Paper Details

Date Published: 20 September 2010
PDF: 12 pages
Proc. SPIE 7804, Developments in X-Ray Tomography VII, 78041G (20 September 2010); doi: 10.1117/12.859437
Show Author Affiliations
Sarper Gürel, Univ. Basel (Switzerland)
Corinne Unold, Univ. Basel (Switzerland)
Hans Deyhle, Univ. Basel (Switzerland)
Georg Schulz, Univ. Basel (Switzerland)
Sebastian Kühl, Univ. Basel (Switzerland)
Belma Saldamli, Technische Univ. München (Germany)
Jutta Tübel, Technische Univ. München (Germany)
Rainer Burgkart, Technische Univ. München (Germany)
Felix Beckmann, GKSS-Forschungszentrum Geesthacht (Germany)
Bert Müller, Univ. Basel (Switzerland)


Published in SPIE Proceedings Vol. 7804:
Developments in X-Ray Tomography VII
Stuart R. Stock, Editor(s)

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