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Proceedings Paper

SERS microscopy: plasmonic nanoparticle probes and biomedical applications
Author(s): M. Gellner; M. Schütz; M. Salehi; J. Packeisen; P. Ströbel; A. Marx; C. Schmuck; S. Schlücker
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Paper Abstract

Nanoparticle probes for use in targeted detection schemes and readout by surface-enhanced Raman scattering (SERS) comprise a metal core, Raman reporter molecules and a protective shell. One design of SERS labels specifically optimized for biomedical applications in conjunction with red laser excitation is based on tunable gold/silver nanoshells, which are completely covered by a self-assembled monolayer (SAM) of Raman reporters. A shell around the SAM-coated metal core stabilizes the colloid and prevents particle aggregation. The optical properties and SERS efficiencies of these plasmonic nanostructures are characterized both experimentally and theoretically. Subsequent bioconjugation of SERS probes to ligands such as antibodies is a prerequisite for the selective detection of the corresponding target molecule via the characteristic Raman signature of the label. Biomedical imaging applications of SERS-labeled antibodies for tumor diagnostics by SERS microscopy are presented, using the localization of the tumor suppressor p63 in prostate tissue sections as an example.

Paper Details

Date Published: 20 August 2010
PDF: 8 pages
Proc. SPIE 7757, Plasmonics: Metallic Nanostructures and Their Optical Properties VIII, 77570M (20 August 2010); doi: 10.1117/12.859253
Show Author Affiliations
M. Gellner, Univ. Osnabrück (Germany)
M. Schütz, Univ. Osnabrück (Germany)
M. Salehi, Univ. Osnabrück (Germany)
J. Packeisen, Medical Ctr., Rheiner Landstraße (Germany)
P. Ströbel, Ruprecht-Karls-Univ. Heidelberg (Germany)
A. Marx, Ruprecht-Karls-Univ. Heidelberg (Germany)
C. Schmuck, Univ. Duisburg-Essen (Germany)
S. Schlücker, Univ. Osnabrück (Germany)

Published in SPIE Proceedings Vol. 7757:
Plasmonics: Metallic Nanostructures and Their Optical Properties VIII
Mark I. Stockman, Editor(s)

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