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Proceedings Paper

Exploring cell dynamics at nanoscale with digital holographic microscopy for diagnostic purposes
Author(s): P. Marquet; D. Boss; J. Kühn; P. Jourdain; C. Depeursinge; P. Magistretti
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Paper Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. The quantitative phase images allow the derivation of highly relevant cell parameters, including dry mass density and spatial distribution. Thanks to a decoupling procedure, cell thickness and intracellular refractive index can be measured separately. Consequently, cell morphology, shape as well as cell membrane fluctuations can be accurately monitor. As far as red blood cell are considered, Mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), two highly relevant clinical parameters, have been measured non-invasively at a single cell level. The DHM nanometric axial and microsecond temporal sensitivities have permitted to measure the red blood cell membrane fluctuations (CMF) over the whole cell surface.

Paper Details

Date Published: 5 May 2010
PDF: 6 pages
Proc. SPIE 7690, Three-Dimensional Imaging, Visualization, and Display 2010 and Display Technologies and Applications for Defense, Security, and Avionics IV, 76900D (5 May 2010); doi: 10.1117/12.855514
Show Author Affiliations
P. Marquet, Univ. of Lausanne (Switzerland)
Ecole Polytechnique Fédérale de Lausanne (Switzerland)
D. Boss, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
J. Kühn, Univ. of Lausanne (Switzerland)
P. Jourdain, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
C. Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
P. Magistretti, Univ. of Lausanne (Switzerland)
Ecole Polytechnique Fédérale de Lausanne (Switzerland)


Published in SPIE Proceedings Vol. 7690:
Three-Dimensional Imaging, Visualization, and Display 2010 and Display Technologies and Applications for Defense, Security, and Avionics IV
Bahram Javidi; John Tudor Thomas; Jung-Young Son; Daniel D. Desjardins, Editor(s)

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