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Proceedings Paper

Peculiarities of RBC aggregation studied by double trap optical tweezers
Author(s): Maria D. Khokhlova; Evgeny V. Lyubin; Alexander G. Zhdanov; Sofia Yu. Rykova; Tatyana N. Krasnova; Irina A. Sokolova; Andrey A. Fedyanin
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Paper Abstract

Aggregation peculiarities of red blood cells (RBCs) in autologous plasma are studied using double trap optical tweezers technique. The positions of RBCs are controlled with submicrometer accuracy by two optical traps formed by strongly focused laser beams (λ=1064 nm). Quantitative measurements of interaction forces between RBCs in pair aggregates are performed. Depending on the RBCs aggregation force, four different end-points of disaggregation induced by optical trap movement are revealed. Analysis of experimental force dependence on the distance between two RBCs during disaggregation is in a good agreement with the model of ring-shaped interaction surfaces of RBCs in pair aggregate. Aggregation velocities measured are shown to be strongly different for healthy and pathologic (System Lupus Erythematosis - SLE) blood samples.

Paper Details

Date Published: 17 May 2010
PDF: 8 pages
Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77150M (17 May 2010); doi: 10.1117/12.854502
Show Author Affiliations
Maria D. Khokhlova, Lomonosov Moscow State Univ. (Russian Federation)
Evgeny V. Lyubin, Lomonosov Moscow State Univ. (Russian Federation)
Alexander G. Zhdanov, Lomonosov Moscow State Univ. (Russian Federation)
Sofia Yu. Rykova, Lomonosov Moscow State Univ. (Russian Federation)
Tatyana N. Krasnova, Lomonosov Moscow State Univ. (Russian Federation)
Irina A. Sokolova, Lomonosov Moscow State Univ. (Russian Federation)
Andrey A. Fedyanin, Lomonosov Moscow State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 7715:
Biophotonics: Photonic Solutions for Better Health Care II
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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