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Proceedings Paper

Digital holography for second harmonic microscopy: application to 3D-tracking of nanoparticles
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Paper Abstract

Retrieval of the amplitude and phase of electromagnetic waves made digital holographic microscopy (DHM) capable of revealing morphological details at ultrahigh resolution in the order of a few nanometers only and precisely measuring the refractive index across a sample (e.g. cell or neuron). In short,DHM added a new dimension to optical imaging,whic h explains why it is such an excellent instrument for metrological,but also for biological applications. We believe that DHM is,b y nature,ideally suited for nonlinear microscopy. In this work,w e review the advantages of DHM for nonlinear microscopy and present its application to determination of the axial position of nonlinear nanoparticles capable of second harmonic generation.

Paper Details

Date Published: 14 May 2010
PDF: 9 pages
Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 771505 (14 May 2010); doi: 10.1117/12.854289
Show Author Affiliations
Etienne Shaffer, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Christian Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 7715:
Biophotonics: Photonic Solutions for Better Health Care II
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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