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Proceedings Paper

Label free detection of DNA hybridization by refractive index tapered fiber biosensor
Author(s): M. I. Zibaii; H. Latifi; E. Ghanati; M. Gholami; S. M. Hosseini
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Paper Abstract

We demonstrate a simple refractive index sensor (RI) sensing system based on a biconical tapered optical fiber (BTOF), which is fabricated by heat pulling method, utilizing a CO2 laser. In this work we explore the application of these sensors for the detection of label free single stranded DNA (ssDNA) in real time. During the experiment, the target ssDNA did not need to be labeled with a fluorescent tag, which is expensive and complicated. The change in output optical transmission of the tapered fiber was recorded for Poly-L-Lysine (PLL) coating, ssDNA probe immobilization and hybridization. The result indicated that due to the hybridization with the complementary target ssDNA on the tapered surface, the RI of surrounding medium changes which leads to changes in the characteristics of the tapered region and change in the output power of the sensor.

Paper Details

Date Published: 18 May 2010
PDF: 9 pages
Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151Z (18 May 2010); doi: 10.1117/12.853974
Show Author Affiliations
M. I. Zibaii, Shahid Beheshti Univ. (Iran, Islamic Republic of)
H. Latifi, Shahid Beheshti Univ. (Iran, Islamic Republic of)
E. Ghanati, Shahid Beheshti Univ. (Iran, Islamic Republic of)
M. Gholami, Shahid Beheshti Univ. (Iran, Islamic Republic of)
S. M. Hosseini, Shahid Beheshti Univ. (Iran, Islamic Republic of)


Published in SPIE Proceedings Vol. 7715:
Biophotonics: Photonic Solutions for Better Health Care II
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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