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Proceedings Paper

High resolution surface plasmon microscopy for cell imaging
Author(s): F. Argoul; K. Monier; T. Roland; J. Elezgaray; L. Berguiga
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Paper Abstract

We introduce a new non-labeling high resolution microscopy method for cellular imaging. This method called SSPM (Scanning Surface Plasmon Microscopy) pushes down the resolution limit of surface plasmon resonance imaging (SPRi) to sub-micronic scales. High resolution SPRi is obtained by the surface plasmon lauching with a high numerical aperture objective lens. The advantages of SPPM compared to other high resolution SPRi's rely on three aspects; (i) the interferometric detection of the back reflected light after plasmon excitation, (ii) the twodimensional scanning of the sample for image reconstruction, (iii) the radial polarization of light, enhancing both resolution and sensitivity. This microscope can afford a lateral resolution of - 150 nm in liquid environment and - 200 nm in air. We present in this paper images of IMR90 fibroblasts obtained with SSPM in dried environment. Internal compartments such as nucleus, nucleolus, mitochondria, cellular and nuclear membrane can be recognized without labelling. We propose an interpretation of the ability of SSPM to reveal high index contrast zones by a local decomposition of the V (Z) function describing the response of the SSPM.

Paper Details

Date Published: 15 May 2010
PDF: 15 pages
Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 771506 (15 May 2010); doi: 10.1117/12.853856
Show Author Affiliations
F. Argoul, Lab. Joliot-Curie and Lab. de Physique, CNRS, Ecole Normale Supérieure de Lyon (France)
K. Monier, Lab. Joliot-Curie, CNRS, Ecole Normale Supérieure de Lyon (France)
T. Roland, Lab. Joliot-Curie and Lab. de Physique, CNRS, Ecole Normale Supérieure de Lyon (France)
J. Elezgaray, Lab. de Microbiologie et Biochimie Appliquée, CNRS, Univ. Bordeaux 1 (France)
L. Berguiga, Lab. Joliot-Curie and Lab. de Physique, CNRS, Ecole Normale Supérieure de Lyon (France)


Published in SPIE Proceedings Vol. 7715:
Biophotonics: Photonic Solutions for Better Health Care II
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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