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Proceedings Paper

Characterization of bone quality in prostate cancer bone metastases using Raman spectroscopy
Author(s): Xiaohong Bi; Chetan Patil; Colm Morrissey; Martine P. Roudier; Anita Mahadevan-Jansen; Jeffry Nyman
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Paper Abstract

Prostate cancer is the most common primary tumor in men, with a high propensity to metastasize to bone. Bone metastases in prostate cancer are associated with active pathologic bone remodeling, leading to increased mortality and morbidity. Detailed characterization of bone metastases is important in the management of prostate cancer. Raman spectroscopy was applied in this study to investigate the structure and composition of metastatic bone in prostate cancer with the ultimate goal of identifying spectral features that are related to the alterations in bone quality as the bone metastases develop. Osteoblastic-, osteolytic- and tumor-absent bone specimens from prostate cancer patients were investigated using bench-top Raman microspectroscopy. Raman derived measurements of collagen mineralization, mineral crystallinity, and carbonate substitution were calculated. The osteolytic lesions demonstrated significantly lower collagen mineralization, determined by phosphate ν1/proline, and higher carbonate substitution than normal and osteoblastic bones. Mineral crystallinity was significantly lower in both blastic and lytic specimens. In addition, a significant increase in the ratio of hydroxyproine: proline was observed in the osteoblastic specimen, indicating an increase in the content of hydroxyproline at the blastic lesions. This study demonstrate that Raman spectroscopy shows promise in determining alterations in osteoblastic and osteolytic bone metastases as well as assessing the response of metastatic bone to therapies.

Paper Details

Date Published: 2 March 2010
PDF: 6 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75484L (2 March 2010); doi: 10.1117/12.853551
Show Author Affiliations
Xiaohong Bi, Vanderbilt Univ. (United States)
Chetan Patil, Vanderbilt Univ. (United States)
Colm Morrissey, Univ. of Washington (United States)
Martine P. Roudier, Univ. of Washington (United States)
Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)
Jeffry Nyman, VA Tennessee Valley Healthcare System (United States)
Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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