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Proceedings Paper

Morphological differences between normal and cancerous mammalian cells via multitechnique microscopic studies
Author(s): Marie-Belle Saab; Elias Estephan; Marta Martin; Nicole Bec; Christian Larroque; Thierry Cloître; Frédéric Cuisinier; Csilla Gergely
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Paper Abstract

Despite of the recent year's important advances in breast cancer biology, there is a continuous demand for new microscopic studies able to provide complementary information on cell shape that is an essential feature of the tumour cells affecting their proliferation and spreading. Understanding the relationship between cell shape and cellular function is important for regulation of cell phenotype modification in particular during cancerogenesis. Utilizing a multitechnique approach, we have investigated the morphological differences of normal human mammalian epithelial cells (HMEC) and cancerous breast epithelial cells (MCF7) cells, both mammalian epithelial cells, but from the same cell type, allowing us to compare them. The goal of our investigation was to combine information on morphological properties of these cells provided by imaging techniques like atomic force microscopy (AFM), brightfield microscopy with in-depth images of microtubules via the multiphoton microscopy (MPM). Cells morphology studies for both cells' types were first carried out using the contact mode AFM which has gained great potential for studying biological systems. Brightfield optical imaging was operated in correlation with the AFM measurements. Topography analyses were performed for living cells as well as fixed ones for both MCF7 and HMEC 184 A1 cells. Living cancerous cells are much softer than normal ones, smaller in shape, and more difficult to manipulate. Photonic responses of fixed cells have been then evaluated by the multiphoton microscopy. Due to light's good penetration depth (IR excitation) in biological samples, MPM has already proved to be a reliable and powerful tool in medical and biological deep tissue imaging. Moreover, MPM provides useful three-dimensional information on the structural and optical properties of the specimen due to its intrinsic optical sectioning resolution. Combination of these microscopic techniques allows us to correlate external cell morphology, with in-depth images provided by the non-linear optical response of microtubules. Understanding cytoskeletal perturbations and particularly, organization of the microtubules can help us to comprehend biological processes in cancer.

Paper Details

Date Published: 17 May 2010
PDF: 10 pages
Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 771518 (17 May 2010); doi: 10.1117/12.853531
Show Author Affiliations
Marie-Belle Saab, Groupe d'Etude des Semi-conducteurs, CNRS, Univ. Montpellier 2 (France)
Elias Estephan, Univ. Montpellier 1 (France)
Marta Martin, Groupe d'Etude des Semi-conducteurs, CNRS, Univ. Montpellier 2 (France)
Nicole Bec, Institut de Recherche en Cancérologie de Montpellier, Univ. Montpellier 1 (France)
Christian Larroque, Institut de Recherche en Cancérologie de Montpellier, Univ. Montpellier 1 (France)
Thierry Cloître, Groupe d'Etude des Semi-conducteurs, CNRS, Univ. Montpellier 2 (France)
Frédéric Cuisinier, Univ. Montpellier 1 (France)
Csilla Gergely, Groupe d'Etude des Semi-conducteurs, CNRS, Univ. Montpellier 2 (France)


Published in SPIE Proceedings Vol. 7715:
Biophotonics: Photonic Solutions for Better Health Care II
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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