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Proceedings Paper

Characterization of aeroallergen of Texas panhandle using scanning and fluorescence microscopy
Author(s): Nabarun Ghosh; Mandy Whiteside; Chris Ridner; Yasemin Celik; C. Saadeh M.D.; Jeff Bennert
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Paper Abstract

Aeroallergens cause serious allergic and asthmatic reactions. Characterizing the aeroallergen provides information regarding the onset, duration, and severity of the pollen season that clinicians use to guide allergen selection for skin testing and treatment. Fluorescence Microscopy has useful approaches to understand the structure and function of the microscopic objects. Prepared slides from the pollen were observed under an Olympus BX40 microscope equipped with FITC and TRITC fluorescent filters, a mercury lamp source, an Olympus DP-70 digital camera connected to the computer with Image Pro 6.0 software. Aeroallergens were viewed, recorded and analyzed with DP Manager using the Image Pro 6.0 software. Photographs were taken at bright field, the fluorescein-isothiocyanate (FITC) filter, and the tetramethylrhodamine (TRITC) filter settings at 40X. A high pressure mercury lamp or UV source was used to excite the storage molecules or proteins which exhibited autofluorescence. The FITC filter reveals the green fluorescent proteins (GFP and EGFP), and the TRITC filter for red fluorescent proteins (DsRed). SEM proved to be useful for observing ultra-structural details like pores, colpi, sulci and ornamentations on the pollen surface. Samples were examined with an SEM (TM-1000) after gold coating and Critical Point Drying. Pollen grains were measured using the TM-1000 imaging software that revealed the specific information on the size of colpi or sulci and the distance between the micro-structures. This information can be used for classification and circumscription in Angiosperm taxonomy. Data were correlated clinical studies established at Allergy A.R.T.S. Clinical Research Laboratory.

Paper Details

Date Published: 7 June 2010
PDF: 9 pages
Proc. SPIE 7729, Scanning Microscopy 2010, 77291X (7 June 2010); doi: 10.1117/12.853145
Show Author Affiliations
Nabarun Ghosh, West Texas A&M Univ. (United States)
Mandy Whiteside, West Texas A&M Univ. (United States)
Chris Ridner, West Texas A&M Univ. (United States)
Yasemin Celik, West Texas A&M Univ. (United States)
C. Saadeh M.D., Allergy A.R.T.S. (United States)
Jeff Bennert, Air Oasis LLP (United States)

Published in SPIE Proceedings Vol. 7729:
Scanning Microscopy 2010
Michael T. Postek; Dale E. Newbury; S. Frank Platek; David C. Joy, Editor(s)

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