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Proceedings Paper

Development of a microfluidic system for measuring HIV-1 viral load
Author(s): Shuqi Wang; Alexander Ip; Feng Xu; Francoise F. Giguel; SangJun Moon; Altug Akay; Daniel R. Kuritzkes; Utkan Demirci
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Paper Abstract

The World Health Organization (WHO) is rapidly expanding antiretroviral treatment (ART) in sub-Saharan countries. However, virological failure of ART is rarely monitored due to the lack of affordable and sustainable viral load assays suitable for resource-limited settings. Here, we report a prototype of a rapid virus detection method based on microfluidic technologies. In this method, HIV-1 particles from 10 μL whole blood were captured by anti-gp120 antibody coated on the microchannel surface and detected by dual fluorescence signals under microscopy. Next, captured HIV-1 particles were counted using the free software, ImageJ (http://rsbweb.nih.gov/ij/). This rapid HIV-1 detection method has potential to be further developed for viral load monitoring at resource-limited settings.

Paper Details

Date Published: 5 May 2010
PDF: 6 pages
Proc. SPIE 7666, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX, 76661H (5 May 2010); doi: 10.1117/12.853132
Show Author Affiliations
Shuqi Wang, Brigham and Women's Hospital (United States)
Alexander Ip, Brigham and Women's Hospital (United States)
Feng Xu, Brigham and Women's Hospital (United States)
Francoise F. Giguel, Massachusetts General Hospital (United States)
SangJun Moon, Brigham and Women's Hospital (United States)
Altug Akay, Brigham and Women's Hospital (United States)
Daniel R. Kuritzkes, Brigham and Women's Hospital (United States)
Utkan Demirci, Brigham and Women's Hospital (United States)
Harvard-MIT Division of Health Sciences and Technology (United States)


Published in SPIE Proceedings Vol. 7666:
Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX
Edward M. Carapezza, Editor(s)

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