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Proceedings Paper

A microfluidic system for semicontinuous bead-based biosensing platforms
Author(s): Hye Won Kim; Young Man Kim; Sung Keun Yoo; Sang Youl Yoon; Jong Hyun Lee; Sung Yang
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Paper Abstract

Conventional bead-based micro immunoassays do not guarantee high reliability and repeatability due to the trapping mechanisms of the systems which cause inconsistency in trapping of cytometric beads. This paper presents a new beadbased micro immunoassay system for semicontinuous assays with high reliability and accuracy in cytometric bead trapping by using the hole-patterned microstructures. Trapping test showed the assembly of single layer of bead array in ordered fashion. In addition that, the μ-immunoassay exhibits that the fluorescence intensity linearly increases in proportion to Biotin-4-Fluorescin (B4F) concentration and a 10 pg/mL appears to be the lowest detectable concentration for B4F after incubation time of 5 minutes.

Paper Details

Date Published: 25 November 2009
PDF: 6 pages
Proc. SPIE 7634, Optical Sensors and Biophotonics, 76340I (25 November 2009); doi: 10.1117/12.853098
Show Author Affiliations
Hye Won Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
Young Man Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
Sung Keun Yoo, Gwangju Institute of Science and Technology (Korea, Republic of)
Sang Youl Yoon, Gwangju Institute of Science and Technology (Korea, Republic of)
Jong Hyun Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Sung Yang, Gwangju Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 7634:
Optical Sensors and Biophotonics
Xingde Li; Qingming Luo; Vasilis Ntziachristos; Yoshiaki Yasuno, Editor(s)

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