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Proceedings Paper

Single dose toxicity study of IRDye 800CW in Sprague-Dawley rats
Author(s): Milton V. Marshall; Daniel Draney; Eva M. Sevick-Muraca; D. Michael Olive
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Paper Abstract

Fluorophore-labeled contrast imaging agents are moving toward clinical use as aids in nodal staging and intraoperative resection of tumors. Near-infrared fluorophores with defined toxicity properties will be needed before these agents can be translated to the clinic. The near-infrared dye IRDye 800CW is frequently used in its N-hydroxysuccinamide (NHS) ester form for labeling these agents. Following conjugation or breakdown of a labeled ligand, excess NHS ester is converted to the carboxylate form. We report here the results of a preliminary toxicity study on IRDye 800CW carboxylate in preparation for its use as a labeling moiety for targeted contrast agents. Male and female Sprague Dawley rats were given a single intravenous or intradermal administration of IRDye 800CW carboxylate; indocyanine green was used as a comparative control. Following administration of varying doses of either the dyes or saline, animals were observed for up to fourteen days during which time, hematological, clinical chemistry, enzymological, and histological testing was performed on animal subgroups. Under the conditions tested, a single administration of IRDye 800CW carboxylate intravenously at dose levels of 1, 5 and 20 mg/kg or 20 mg/kg intradermally produced no pathological evidence of toxicity. A dose of 20 mg/kg was identified as the NOAEL (no observed adverse effect level) following IV or ID routes of administration of IRDye 800CW.

Paper Details

Date Published: 16 February 2010
PDF: 11 pages
Proc. SPIE 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, 757609 (16 February 2010); doi: 10.1117/12.852783
Show Author Affiliations
Milton V. Marshall, Baylor College of Medicine (United States)
Daniel Draney, LI-COR Biosciences (United States)
Eva M. Sevick-Muraca, Baylor College of Medicine (United States)
D. Michael Olive, LI-COR Biosciences (United States)


Published in SPIE Proceedings Vol. 7576:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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