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Proceedings Paper

Quantum-dot-based quantitative identification of pathogens in complex mixture
Author(s): Sun Hee Lim; Felix Bestwater; Philippe Buchy; Sek Mardy; Alexey Dan Chin Yu
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Paper Abstract

In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-, and 100-mer target DNA with both probes was analyzed and quantified by flow cytometry and fluorescence microscopy on the scale of single particles. The following steps were used in the assay: (i) target selection by MP probes and (ii) target detection by QD probes. Hybridization efficiency between MP conjugated probes and target DNA hybrids was controlled by a fluorescent dye specific for nucleic acids. Fluorescence was detected by flow cytometry to distinguish differences in oligo sequences as short as 25-mer capturing in target DNA and by gel-electrophoresis in the case of QD probes. This report shows that effective manipulation and control of micro- and nanoparticles in hybridization assays is possible.

Paper Details

Date Published: 18 February 2010
PDF: 11 pages
Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 75750M (18 February 2010); doi: 10.1117/12.851050
Show Author Affiliations
Sun Hee Lim, Institut Pasteur Korea (Korea, Republic of)
Ctr. for Neglected Diseases Drug Discovery, Institut Pasteur Korea (Korea, Republic of)
Felix Bestwater, Institut Pasteur Korea (Korea, Republic of)
Philippe Buchy, Institut Pasteur Cambodia (Cambodia)
Sek Mardy, Institut Pasteur Cambodia (Cambodia)
Alexey Dan Chin Yu, Institut Pasteur in Cambodia (Korea, Republic of)
Korean Advanced Institute of Science and Technology (Korea, Republic of)

Published in SPIE Proceedings Vol. 7575:
Colloidal Quantum Dots for Biomedical Applications V
Marek Osinski; Wolfgang J. Parak; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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