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Proceedings Paper

Parallel microfluidic arrays for SPRi detection
Author(s): Eric Ouellet; Christopher Lausted; Tao Lin; Cheng-Wei Yang; Leroy Hood; Eric T. Lagally
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Paper Abstract

Surface Plasmon Resonance imaging (SPRi) is a label-free technique for the quantitation of binding affinities and concentrations for a wide variety of target molecules. Although SPRi is capable of determining binding constants for multiple ligands in parallel, current commercial instruments are limited to a single analyte stream and a limited number of ligand spots. Measurement of target concentration also requires the serial introduction of different target concentrations; such repeated experiments are conducted manually and are therefore time-intensive. Likewise, the equilibrium determination of concentration for known binding affinity requires long times due to diffusion-limited kinetics to a surface-immobilized ligand. We have developed an integrated microfluidic array using soft lithography techniques for SPRi-based detection and determination of binding affinities for DNA aptamers against human alphathrombin. The device consists of 264 element-addressable chambers of 700 pL each isolated by microvalves. The device also contains a dilution network for simultaneous interrogation of up to six different target concentrations, further speeding detection times. The element-addressable design of the array allows interrogation of multiple ligands against multiple targets, and analytes from individual chambers may be collected for downstream analysis.

Paper Details

Date Published: 28 April 2010
PDF: 9 pages
Proc. SPIE 7682, Photonic Microdevices/Microstructures for Sensing II, 76820F (28 April 2010); doi: 10.1117/12.850522
Show Author Affiliations
Eric Ouellet, The Univ. of British Columbia (Canada)
Christopher Lausted, Institute for Systems Biology (United States)
Tao Lin, The Univ. of British Columbia (Canada)
Cheng-Wei Yang, The Univ. of British Columbia (Canada)
Leroy Hood, Institute for Systems Biology (United States)
Eric T. Lagally, The Univ. of British Columbia (Canada)

Published in SPIE Proceedings Vol. 7682:
Photonic Microdevices/Microstructures for Sensing II
Xudong Fan; Hai Xiao; Anbo Wang, Editor(s)

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