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Proceedings Paper

Development of an integrated system for rapid detection of biological agents
Author(s): Hideyuki Terazono; Hiroyuki Takei; Masahito Hayashi; Akihiro Hattori; Kenji Yasuda
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Paper Abstract

Weaponized biological agents are as great a threat as nuclear or chemical weapons. They must be detected at the earliest stage to prevent diffusion because once these agents are dispersed into the air, the rapidly decreasing concentration makes detection more of a challenge. Polymerase chain reaction (PCR) is a common method to create copies of a specific target region of a DNA sequence and to produce large quantities of DNA molecules. A few DNA molecules are rapidly amplified by PCR into billions of copies. While PCR is a powerful technique and is capable of countering new threats relatively easily, it is plagued by the number of processes necessary. Therefore, we have developed an integrated PCR system for rapid detection of biological agents captured from the air. Each processing function is performed by a dedicated module, and reduction in the process time has been made the top priority, without loss in the signal/noise ratio of the total system. Agents can be identified within 15 min from capture. A fully automated operation protects operators from exposure to potentially highly lethal samples.

Paper Details

Date Published: 5 May 2010
PDF: 10 pages
Proc. SPIE 7665, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XI, 766503 (5 May 2010); doi: 10.1117/12.849830
Show Author Affiliations
Hideyuki Terazono, Kanagawa Academy of Science and Technology (Japan)
Hiroyuki Takei, Tokyo Medical and Dental Univ. (Japan)
Toyo Univ. (Japan)
Masahito Hayashi, Kanagawa Academy of Science and Technology (Japan)
Akihiro Hattori, On-chip Cellomics Consortium Co., Ltd. (Japan)
Kenji Yasuda, Kanagawa Academy of Science and Technology (Japan)
Tokyo Medical and Dental Univ. (Japan)


Published in SPIE Proceedings Vol. 7665:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XI
Augustus Way Fountain III; Patrick J. Gardner, Editor(s)

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