Share Email Print
cover

Proceedings Paper

Biological applications of fluorescence lifetime imaging beyond microscopy
Author(s): Walter J. Akers; Mikhail Y. Berezin; Hyeran Lee; Kevin Guo; Adah Almutairi; Jean M. J. Fréchet; Georg M. Fischer; Ewald Daltrozzo; Samuel Achilefu
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Fluorescence lifetime is a relatively new contrast mechanism for optical imaging in living subjects that relies on intrinsic properties of fluorophores rather than concentration dependent intensity. Drawing upon the success of fluorescence lifetime imaging microscopy (FLIM) for investigation of protein-protein interactions and intracellular physiology, in vivo fluorescence lifetime imaging (FLI) promises to dramatically increase the utility of fluorescencebased imaging in preclinical and clinical applications. Intrinsic fluorescence lifetime measurements in living tissues can distinguish pathologies such as cancer from healthy tissue. Unfortunately, intrinsic FLT contrast is limited to superficial measurements. Conventional intensity-based agents have been reported for measuring these phenomena in vitro, but translation into living animals is difficult due to optical properties of tissues. For this reason, contrast agents that can be detected in the near infrared (NIR) wavelengths are being developed by our lab and others to enhance the capabilities of this modality. FLT is less affected by concentration and thus is better for detecting small changes in physiology, as long as sufficient fluorescence signal can be measured. FLT can also improve localization of signals for improved deep tissue imaging. Examples of the utility of exogenous contrast agents will be discussed, including applications in monitoring physiologic functions, controlled drug release and cancer biology. Instrumentation for FLI will also be discussed, including planar and diffuse optical imaging in time and frequency domains. Future applications will also be discussed that are being developed in this exciting field that complement other optical modalities.

Paper Details

Date Published: 17 February 2010
PDF: 9 pages
Proc. SPIE 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, 757612 (17 February 2010); doi: 10.1117/12.849453
Show Author Affiliations
Walter J. Akers, Washington Univ. School of Medicine in St. Louis (United States)
Mikhail Y. Berezin, Washington Univ. School of Medicine in St. Louis (United States)
Hyeran Lee, Washington Univ. School of Medicine in St. Louis (United States)
Kevin Guo, Washington Univ. School of Medicine in St. Louis (United States)
Adah Almutairi, Univ. of California, San Diego (United States)
Jean M. J. Fréchet, Univ. of California, Berkeley (United States)
Georg M. Fischer, Univ. Konstanz (Germany)
Ewald Daltrozzo, Univ. Konstanz (Germany)
Samuel Achilefu, Washington Univ. School of Medicine in St. Louis (United States)


Published in SPIE Proceedings Vol. 7576:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

© SPIE. Terms of Use
Back to Top