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Proceedings Paper

Fully automated corneal endothelial morphometry of images captured by clinical specular microscopy
Author(s): Curry Bucht; Per Söderberg; Göran Manneberg
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Paper Abstract

The corneal endothelium serves as the posterior barrier of the cornea. Factors such as clarity and refractive properties of the cornea are in direct relationship to the quality of the endothelium. The endothelial cell density is considered the most important morphological factor of the corneal endothelium. Pathological conditions and physical trauma may threaten the endothelial cell density to such an extent that the optical property of the cornea and thus clear eyesight is threatened. Diagnosis of the corneal endothelium through morphometry is an important part of several clinical applications. Morphometry of the corneal endothelium is presently carried out by semi automated analysis of pictures captured by a Clinical Specular Microscope (CSM). Because of the occasional need of operator involvement, this process can be tedious, having a negative impact on sampling size. This study was dedicated to the development and use of fully automated analysis of a very large range of images of the corneal endothelium, captured by CSM, using Fourier analysis. Software was developed in the mathematical programming language Matlab. Pictures of the corneal endothelium, captured by CSM, were read into the analysis software. The software automatically performed digital enhancement of the images, normalizing lights and contrasts. The digitally enhanced images of the corneal endothelium were Fourier transformed, using the fast Fourier transform (FFT) and stored as new images. Tools were developed and applied for identification and analysis of relevant characteristics of the Fourier transformed images. The data obtained from each Fourier transformed image was used to calculate the mean cell density of its corresponding corneal endothelium. The calculation was based on well known diffraction theory. Results in form of estimated cell density of the corneal endothelium were obtained, using fully automated analysis software on 292 images captured by CSM. The cell density obtained by the fully automated analysis was compared to the cell density obtained from classical, semi-automated analysis and a relatively large correlation was found.

Paper Details

Date Published: 2 March 2010
PDF: 8 pages
Proc. SPIE 7550, Ophthalmic Technologies XX, 75501E (2 March 2010); doi: 10.1117/12.849255
Show Author Affiliations
Curry Bucht, St. Erik Eye Hospital, Karolinska Institutet (Sweden)
Per Söderberg, Uppsala Univ. (Sweden)
Göran Manneberg, Royal Institute of Technology (Sweden)

Published in SPIE Proceedings Vol. 7550:
Ophthalmic Technologies XX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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