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Proceedings Paper

Laser induced fluorescence lifetime characterization of Bacillus endospore species using time correlated single photon counting analysis with the multi-exponential fit method
Author(s): Clint Smith; Jarrod Edwards; Andmorgan Fisher
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Paper Abstract

Rapid detection of biological material is critical for determining presence/absence of bacterial endospores within various investigative programs. Even more critical is that if select material tests positive for bacillus endospores then tests should provide data at the species level. Optical detection of microbial endospore formers such as Bacillus sp. can be heavy, cumbersome, and may only identify at the genus level. Data provided from this study will aid in characterization needed by future detection systems for further rapid breakdown analysis to gain insight into a more positive signature collection of Bacillus sp. Literature has shown that fluorescence spectroscopy of endospores could be statistically separated from other vegetative genera, but could not be separated among one another. Results of this study showed endospore species separation is possible using laser-induce fluorescence with lifetime decay analysis for Bacillus endospores. Lifetime decays of B. subtilis, B. megaterium, B. coagulans, and B. anthracis Sterne strain were investigated. Using the Multi-Exponential fit method data showed three distinct lifetimes for each species within the following ranges, 0.2-1.3 ns; 2.5-7.0 ns; 7.5-15.0 ns, when laser induced at 307 nm. The four endospore species were individually separated using principle component analysis (95% CI).

Paper Details

Date Published: 4 May 2010
PDF: 6 pages
Proc. SPIE 7687, Active and Passive Signatures, 76870B (4 May 2010); doi: 10.1117/12.849219
Show Author Affiliations
Clint Smith, Army Engineer Research and Development Ctr. (United States)
Jarrod Edwards, Army Engineer Research and Development Ctr. (United States)
Andmorgan Fisher, Army Engineer Research and Development Ctr. (United States)

Published in SPIE Proceedings Vol. 7687:
Active and Passive Signatures
G. Charmaine Gilbreath; Chadwick T. Hawley, Editor(s)

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