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Proceedings Paper

Cellular spectroscopy and multi-angle light scattering by flow cytometry: optical test bench as a developmental tool
Author(s): Gordon Wiegand; Itzhak Avital
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Paper Abstract

We designed and fabricated a flow cytometry work bench for the purpose of testing optical schemes utilized in two specialized flow cytometry techniques. Fluorescent spectroscopy and multi-angle scattering of laser light has potential for generating novel classes of cell population information. The acquisition of full fluorescence spectrum of dye bound to living cells may associate specific function to spectral shift. Similarly, detection of 2 discrete angles of light scatter was shown to describe internal structures of cells based on refractive and diffractive properties. We utilized a flow cytometry electro- cells based on refractive and diffractive properties. We utilized a flow cytometry electro- optical test bench to develop and describe alternate light collection schemes for these two techniques. Mouse bone marrow was chosen as the subject of analysis because it contains a consistent mix of cells that possess distinct biophysical properties. More importantly, these cells are the source of various biological systems that are targeted by environmental stress and clinical disease.

Paper Details

Date Published: 25 February 2010
PDF: 9 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75681J (25 February 2010); doi: 10.1117/12.849163
Show Author Affiliations
Gordon Wiegand, National Institutes of Health (United States)
Itzhak Avital, National Institutes of Health (United States)


Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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