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Proceedings Paper

DNA probe immobilisation using 3D hydrogel matrix in a hollow core Photonic Crystal Fibre
Author(s): M. Rutowska; F. Kivlehan; E. Moore; D. Brennan; P. Galvin; F. C. Garcia Gunning
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Paper Abstract

In this paper, we show for the first time the integration of a 3D hydrogel matrix within a hollow core Photonic Crystal Fibre (HC-PCF) and we also show the fluorescence propagation of a Cy5-labelled DNA probe immobilisation within the hydrogel formed in two different types of HC-PCF. The 3D hydrogel matrix is designed to bind with the amino-groups of biomolecules, providing higher sensitivity and selectivity than the standard 2D coverage, enabling a greater number of probe molecules to be available per unit area. The HC-PCFs, on the other hand, are designed to maximise the capture of fluorescence to improve sensitivity, providing long interaction lengths, being easily integrated with light sources and detectors, and it can also be implemented in point-of-care or remote systems.

Paper Details

Date Published: 14 October 2009
PDF: 4 pages
Proc. SPIE 7503, 20th International Conference on Optical Fibre Sensors, 750382 (14 October 2009); doi: 10.1117/12.848771
Show Author Affiliations
M. Rutowska, Tyndall National Institute, Univ. College Cork (Ireland)
F. Kivlehan, Tyndall National Institute, Univ. College Cork (Ireland)
E. Moore, Tyndall National Institute, Univ. College Cork (Ireland)
D. Brennan, Tyndall National Institute, Univ. College Cork (Ireland)
P. Galvin, Tyndall National Institute, Univ. College Cork (Ireland)
F. C. Garcia Gunning, Tyndall National Institute, Univ. College Cork (Ireland)


Published in SPIE Proceedings Vol. 7503:
20th International Conference on Optical Fibre Sensors
Julian D. C. Jones, Editor(s)

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