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Proceedings Paper

Wide-field and high-resolution optical imaging for early detection of oral neoplasia
Author(s): Mark C. Pierce; Richard A. Schwarz; Kelsey Rosbach; Darren Roblyer; Tim Muldoon; Michelle D. Williams; Adel K. El-Naggar; Ann M. Gillenwater; Rebecca Richards-Kortum
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Paper Abstract

Current procedures for oral cancer screening typically involve visual inspection of the entire tissue surface at risk under white light illumination. However, pre-cancerous lesions can be difficult to distinguish from many benign conditions when viewed under these conditions. We have developed wide-field (macroscopic) imaging system which additionally images in cross-polarized white light, narrowband reflectance, and fluorescence imaging modes to reduce specular glare, enhance vascular contrast, and detect disease-related alterations in tissue autofluorescence. We have also developed a portable system to enable high-resolution (microscopic) evaluation of cellular features within the oral mucosa in situ. This system is a wide-field epi-fluorescence microscope coupled to a 1 mm diameter, flexible fiber-optic imaging bundle. Proflavine solution was used to specifically label cell nuclei, enabling the characteristic differences in N/C ratio and nuclear distribution between normal, dysplastic, and cancerous oral mucosa to be quantified. This paper discusses the technical design and performance characteristics of these complementary imaging systems. We will also present data from ongoing clinical studies aimed at evaluating diagnostic performance of these systems for detection of oral neoplasia.

Paper Details

Date Published: 2 March 2010
PDF: 5 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75481U (2 March 2010); doi: 10.1117/12.848347
Show Author Affiliations
Mark C. Pierce, Rice Univ. (United States)
Richard A. Schwarz, Rice Univ. (United States)
Kelsey Rosbach, Rice Univ. (United States)
Darren Roblyer, Rice Univ. (United States)
Tim Muldoon, Rice Univ. (United States)
Michelle D. Williams, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Adel K. El-Naggar, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Ann M. Gillenwater, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Rebecca Richards-Kortum, Rice Univ. (United States)

Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong; Nikiforos Kollias; Henry Hirschberg; Kenton W. Gregory; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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