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Proceedings Paper

dSTORM: real-time subdiffraction-resolution fluorescence imaging with organic fluorophores
Author(s): Mark Schüttpelz; Steve Wolter; Sebastian van de Linde; Mike Heilemann; Markus Sauer
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Paper Abstract

In the recent past, a variety of methods have been developed to circumvent the diffraction barrier of light which restricts optical resolution to about 200 nm in the image plane. Single-molecule based photoswitching microscopy such as direct stochastic optical reconstruction microscopy (dSTORM) has been successfully implemented for subdiffraction-resolution fluorescence imaging. The major drawback of this technique has been that the reconstruction of subdiffraction-resolution images requires substantially more time than the actual experiment and prevented real-time imaging. Here we present a new computational algorithm enabling subdiffraction-resolution fast imaging of cellular structures with ~20 nm optical resolution in less than 10 seconds.

Paper Details

Date Published: 25 February 2010
PDF: 7 pages
Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 75710V (25 February 2010); doi: 10.1117/12.848105
Show Author Affiliations
Mark Schüttpelz, Univ. Bielefeld (Germany)
Steve Wolter, Univ. Bielefeld (Germany)
Julius-Maximilians-Univ. Würzburg (Germany)
Sebastian van de Linde, Univ. Bielefeld (Germany)
Julius-Maximilians-Univ. Würzburg (Germany)
Mike Heilemann, Univ. Bielefeld (Germany)
Markus Sauer, Univ. Bielefeld (Germany)
Julius-Maximilians-Univ. Würzburg (Germany)

Published in SPIE Proceedings Vol. 7571:
Single Molecule Spectroscopy and Imaging III
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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