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Proceedings Paper

Endoscopic ICG perfusion imaging for flap transplants: technical development
Author(s): Herbert Stepp; Hilmar Schachenmayr; André Ehrhardt; Werner Göbel; Sven Zhorzel; Christian Stephan Betz
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Paper Abstract

Objective: Following tumour surgery in the head and neck region, skin flap transplants are usually required to cover the resection area. The purpose of the development was to provide a simple and reliable means to assess whether the transplanted flap is sufficiently perfused. Methods: Fluorescence of intravenously injected Indocyanine green (ICG) was detected with a slightly modified 3-chip CCD camera. Appropriately coated optical filters allow for excitation of ICG with NIR light and detection of NIR ICGfluorescence with the blue channel of the camera. In addition, low intensities of white light can be transmitted to allow for simultaneous display of a remission image in the green and red channels of the camera. Further processing was performed with a LabVIEW program. Results: A satisfactory white light image (red, green and blue display (RGB)) could be calculated from the remission images recorded with the green and red channels of the camera via a look-up table. The look-up table was programmed to provide an optimized blue intensity value for each combination of red and green values. This was generated using a reference image. Implementation of image tracking and intensity measurements in regions of interest (ROIs) in the images is useful to reliably monitor perfusion kinetics of flap and adjacent normal tissue.

Paper Details

Date Published: 2 March 2010
PDF: 5 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75482A (2 March 2010); doi: 10.1117/12.847857
Show Author Affiliations
Herbert Stepp, Univ. Clinic Munich (Germany)
Hilmar Schachenmayr, Univ. Clinic Munich (Germany)
Karl Storz GmbH & Co. KG (Germany)
André Ehrhardt, Karl Storz GmbH & Co. KG (Germany)
Werner Göbel, Karl Storz GmbH & Co. KG (Germany)
Sven Zhorzel, Univ. Clinic Munich (Germany)
Christian Stephan Betz, Univ. Clinic Munich (Germany)

Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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