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Proceedings Paper

Small animal ocular biometry using optical coherence tomography
Author(s): Marco Ruggeri; Omer Kocaoglu; Stephen Uhlhorn; David Borja; Raksha Urs; Tsung-Han Chou; Vittorio Porciatti; Jean-Marie Parel; Fabrice Manns
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Paper Abstract

A custom-built OCT system was used to obtain images of the whole mouse eye. We developed a semi-automated segmentation method to detect the boundaries of the anterior and posterior corneal, lens and retinal surfaces as well as the anterior surface of the iris. The radii of curvature of the surfaces were calculated using a conic section fit of each boundary. Image distortions due to refraction of the OCT beam at the successive boundaries were corrected using a ray-tracing algorithm. Corrected ocular distances, radii of curvature of the cornea and lens surfaces, and anterior chamber angle were obtained on 3 C57BL/6J mice. In vivo imaging of the whole eye, segmentation, conic function fits and correction were successful in all three animals. The posterior lens surface of one mouse could not be fit accurately with a conic section. Biometric parameters of C57BL/6J mice compared well with previous published data obtained from histological sections. The study demonstrates the feasibility of quantitative in vivo biometry of mouse models.

Paper Details

Date Published: 2 March 2010
PDF: 6 pages
Proc. SPIE 7550, Ophthalmic Technologies XX, 755016 (2 March 2010); doi: 10.1117/12.846937
Show Author Affiliations
Marco Ruggeri, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine (United States)
Biomedical Optics Lab., Univ. of Miami (United States)
Omer Kocaoglu, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine (United States)
Biomedical Optics Lab., Univ. of Miami (United States)
Stephen Uhlhorn, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine (United States)
Biomedical Optics Lab., Univ. of Miami (United States)
David Borja, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine (United States)
Biomedical Optics Lab., Univ. of Miami (United States)
Raksha Urs, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine (United States)
Biomedical Optics Lab., Univ. of Miami (United States)
Tsung-Han Chou, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine (United States)
Biomedical Optics Lab., Univ. of Miami (United States)
Vittorio Porciatti, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine (United States)
Jean-Marie Parel, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine (United States)
Biomedical Optics Lab., Univ. of Miami (United States)
Vision Cooperative Research Ctr. (Australia)
Fabrice Manns, Bascom Palmer Eye Institute, Univ. of Miami Miller School of Medicine (United States)
Biomedical Optics Lab., Univ. of Miami (United States)


Published in SPIE Proceedings Vol. 7550:
Ophthalmic Technologies XX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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