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Proceedings Paper

High-throughput single-molecule fluorescence spectroscopy using parallel detection
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Paper Abstract

Solution-based single-molecule fluorescence spectroscopy is a powerful new experimental approach with applications in all fields of natural sciences. The basic concept of this technique is to excite and collect light from a very small volume (typically femtoliter) and work in a concentration regime resulting in rare burst-like events corresponding to the transit of a single-molecule. Those events are accumulated over time to achieve proper statistical accuracy. Therefore the advantage of extreme sensitivity is somewhat counterbalanced by a very long acquisition time. One way to speed up data acquisition is parallelization. Here we will discuss a general approach to address this issue, using a multispot excitation and detection geometry that can accommodate different types of novel highly-parallel detector arrays. We will illustrate the potential of this approach with fluorescence correlation spectroscopy (FCS) and single-molecule fluorescence measurements obtained with different novel multipixel single-photon counting detectors.

Paper Details

Date Published: 23 January 2010
PDF: 12 pages
Proc. SPIE 7608, Quantum Sensing and Nanophotonic Devices VII, 76082D (23 January 2010); doi: 10.1117/12.846784
Show Author Affiliations
X. Michalet, Univ. of California, Los Angeles (United States)
R. A. Colyer, Univ. of California, Los Angeles (United States)
G. Scalia, Univ. of California, Los Angeles (United States)
T. Kim, Nesher Technologies Inc. (United States)
Moran Levi, Univ. of California, Los Angeles (United States)
Daniel Aharoni, Univ. of California, Los Angeles (United States)
Adrian Cheng, Univ. of California, Los Angeles (United States)
F. Guerrieri, Politecnico di Milano (Italy)
Katsushi Arisaka, Univ. of California, Los Angeles (United States)
Jacques Millaud, Univ. of California, Los Angeles (United States)
I. Rech, Politecnico di Milano (Italy)
D. Resnati, Politecnico di Milano (Italy)
S. Marangoni, Politecnico di Milano (Italy)
A. Gulinatti, Politecnico di Milano (Italy)
M. Ghioni, Politecnico di Milano (Italy)
S. Tisa, Politecnico di Milano (Italy)
F. Zappa, Politecnico di Milano (Italy)
S. Cova, Politecnico di Milano (Italy)
S. Weiss, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 7608:
Quantum Sensing and Nanophotonic Devices VII
Manijeh Razeghi; Rengarajan Sudharsanan; Gail J. Brown, Editor(s)

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