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Proceedings Paper

Imaging retinal nerve fiber bundles at ultrahigh-speed and ultrahigh-resolution using OCT with adaptive optics
Author(s): Omer Pars Kocaoglu; Barry Cense; Qiang Wang; Jeremy Bruestle; Jason Besecker; Weihua Gao; Ravi Jonnal; Donald T. Miller
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Paper Abstract

Ultrahigh speed line scan detectors based on CMOS technology have been recently demonstrated in ultrahigh resolution spectral-domain optical coherence tomography (UHR-SD-OCT) for retinal imaging. While successful, fundamental tradeoffs exist been image acquisition time, image sampling density, and sensitivity, all of which impact the extent of motion artifacts, visualization of fine spatial detail, and detection of faint reflections. Here we investigate these tradeoffs for imaging retinal nerve fiber bundles (RNFBs) using UHR-SD-OCT with adaptive optics (AO). Volume scans of 3°x3° and 1.5°x1.5° were acquired at retinal locations of 3° nasal and 6° superior to the fovea on a healthy subject. Dynamic AO compensation across a 6 mm pupil provided near-diffraction-limited performance. The acquisition rates were 22.5k lines/s and 125k lines/s with A-lines spaced at 0.9 μm and 1.8 μm and B-scans at 1.8 μm and 9 μm. Focus was optimized for visualizing the retinal nerve fiber bundles (RNFBs). En face projection and crosssectional views of the RNFBs were extracted from the volumes and compared to images acquired with established conventional CCD-based line-scan camera. The projection view was found highly sensitive to eye motion artifacts, yet could only be partially compensated with coarser sampling, since fine sampling was necessary to observe the microscopic features in the RNFBs. For the cross-sectional view, speckle noise rather than eye motion artifacts limited bundle clarity. The highest B-scan density (1.8 μm spacing) coupled with B-scan averaging proved the best combination. Regardless of view, the higher line rate provided better RNFB clarity.

Paper Details

Date Published: 2 March 2010
PDF: 5 pages
Proc. SPIE 7550, Ophthalmic Technologies XX, 755010 (2 March 2010); doi: 10.1117/12.846585
Show Author Affiliations
Omer Pars Kocaoglu, Indiana Univ. (United States)
Barry Cense, Utsunomiya Univ. (Japan)
Qiang Wang, Indiana Univ. (United States)
Jeremy Bruestle, Indiana Univ. (United States)
Jason Besecker, Indiana Univ. (United States)
Weihua Gao, Indiana Univ. (United States)
Ravi Jonnal, Indiana Univ. (United States)
Donald T. Miller, Indiana Univ. (United States)

Published in SPIE Proceedings Vol. 7550:
Ophthalmic Technologies XX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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