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Proceedings Paper

Multispectral autofluorescence lifetime imaging of RPE cells using two-photon excitation
Author(s): Lingling Zhao; Danni Chen; Jing Qi; Junle Qu
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Paper Abstract

In this paper, we present our investigation on multispectral autofluorescence lifetime imaging of RPE cells using two-photon excitation. Morphological characters of RPE cells are obtained with high spatial resolution. Different autofluorescence lifetime parameters have been compared at different emission bands. Spatial distribution of dominant endogenous fluorophores in RPE cells, such as FAD, A2E and AGE etc have been obtained by the analysis of τm and a1/a2 ratio in the whole emission spectrum.

Paper Details

Date Published: 26 February 2010
PDF: 5 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692B (26 February 2010); doi: 10.1117/12.845952
Show Author Affiliations
Lingling Zhao, Shenzhen Univ. (China)
Danni Chen, Shenzhen Univ. (China)
Jing Qi, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)


Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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