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Proceedings Paper

Hardware and software system for automatic microemulsion assay evaluation by analysis of optical properties
Author(s): Ulf Maeder; Thomas Schmidts; Jan-Michael Burg; Johannes T. Heverhagen; Frank Runkel; Martin Fiebich
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Paper Abstract

A new hardware device called Microemulsion Analyzer (MEA), which facilitates the preparation and evaluation of microemulsions, was developed. Microemulsions, consisting of three phases (oil, surfactant and water) and prepared on deep well plates according to the PDMPD method can be automatically evaluated by means of the optical properties. The ratio of ingredients to form a microemulsion strongly depends on the properties and the amounts of the used ingredients. A microemulsion assay is set up on deep well plates to determine these ratios. The optical properties of the ingredients change from turbid to transparent as soon as a microemulsion is formed. The MEA contains a frame and an imageprocessing and analysis algorithm. The frame itself consists of aluminum, an electro luminescent foil (ELF) and a camera. As the frame keeps the well plate at the correct position and angle, the ELF provides constant illumination of the plate from below. The camera provides an image that is processed by the algorithm to automatically evaluate the turbidity in the wells. Using the determined parameters, a phase diagram is created that visualizes the information. This build-up can be used to analyze microemulsion assays and to get results in a standardized way. In addition, it is possible to perform stability tests of the assay by creating special differential stability diagrams after a period of time.

Paper Details

Date Published: 9 March 2010
PDF: 9 pages
Proc. SPIE 7626, Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging, 762625 (9 March 2010); doi: 10.1117/12.845489
Show Author Affiliations
Ulf Maeder, Univ. of Applied Sciences Giessen-Friedberg (Germany)
Thomas Schmidts, Univ. of Applied Sciences Giessen-Friedberg (Germany)
Jan-Michael Burg, Univ. of Applied Sciences Giessen-Friedberg (Germany)
Johannes T. Heverhagen, Philipps-Univ. Marburg (Germany)
Frank Runkel, Univ. of Applied Sciences Giessen-Friedberg (Germany)
Martin Fiebich, Univ. of Applied Sciences Giessen-Friedberg (Germany)


Published in SPIE Proceedings Vol. 7626:
Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging
Robert C. Molthen; John B. Weaver, Editor(s)

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