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Proceedings Paper

Dynamic and label-free high-throughput detection of biomolecular interactions based on phase-shift interferometry
Author(s): Qiang Li; Guoliang Huang; Wupeng Gan; Shengyi Chen
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Paper Abstract

Biomolecular interactions can be detected by many established technologies such as fluorescence imaging, surface plasmon resonance (SPR)[1-4], interferometry and radioactive labeling of the analyte. In this study, we have designed and constructed a label-free, real-time sensing platform and its operating imaging instrument that detects interactions using optical phase differences from the accumulation of biological material on solid substrates. This system allows us to monitor biomolecular interactions in real time and quantify concentration changes during micro-mixing processes by measuring the changes of the optical path length (OPD). This simple interferometric technology monitors the optical phase difference resulting from accumulated biomolecular mass. A label-free protein chip that forms a 4×4 probe array was designed and fabricated using a commercial microarray robot spotter on solid substrates. Two positive control probe lines of BSA (Bovine Serum Albumin) and two experimental human IgG and goat IgG was used. The binding of multiple protein targets was performed and continuously detected by using this label-free and real-time sensing platform.

Paper Details

Date Published: 29 October 2009
PDF: 10 pages
Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75190H (29 October 2009); doi: 10.1117/12.845392
Show Author Affiliations
Qiang Li, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Guoliang Huang, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Wupeng Gan, National Engineering Research Ctr. for Beijing Biochip Technology (China)
Shengyi Chen, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)


Published in SPIE Proceedings Vol. 7519:
Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Pengcheng Li; Ling Fu, Editor(s)

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