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Proceedings Paper

Quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging
Author(s): Pierre Bon; Benoit Wattellier; Serge Monneret; Didier Marguet
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Paper Abstract

Phase imaging with a high-resolution wavefront sensor is a useful setup for biological imaging. Our setup is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. We propose here to study long-time duration imaging on different type of adherent cells: green monkey kidney COS7 cells, human breast epithelial MCF10A cells, and human breast cancer derived from MDA-231 cells. This study permits a enhanced visualization of the whole cell life at different levels of confluence. Post treatments on phase-shift images are proposed and become very interesting for enhanced visualization of small details and thresholding.

Paper Details

Date Published: 25 February 2010
PDF: 6 pages
Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700E (25 February 2010); doi: 10.1117/12.844930
Show Author Affiliations
Pierre Bon, Aix-Marseille Univ., Ecole Centrale Marseille, CNRS, Institut Fresnel (France)
PHASICS S.A. (France)
Benoit Wattellier, PHASICS S.A. (France)
Serge Monneret, Aix-Marseille Univ., Ecole Centrale Marseille, CNRS, Institut Fresnel (France)
Didier Marguet, Ctr. Immunologique de Marseille Luminy, CNRS, Univ.de Provence (France)


Published in SPIE Proceedings Vol. 7570:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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