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Proceedings Paper

Sample to answer: a fully integrated nucleic acid identification system for bacteria monitoring
Author(s): Jungkyu Kim; John Elsnab; Michael Johnson; Bruce K. Gale
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Paper Abstract

A fully integrated microfluidic system was developed and incorporates an EC-MWCNT (electrochemical multiwalled carbon nanotube) sensor for the detection of bacteria. Sample metering, reagent metering and delivery was implemented with microvalves and pumps embedded inside the microfluidic system. The nucleic acid extraction was performed using microchannels controlled using automated platforms and a disposable microfluidic silica cartridge. The target samples were flowed and hybridized with probe ssDNA (single strand DNA) across the MWCNT-EC sensor (built on a silicon chip), which was embedded in a microfluidic cell. The 9-pad sensor was scanned before and after hybridization to measure the quantity of RNA (Ribonucleic acid) bound to the array surface. A rapid and accurate sample-in answer-out nucleic acid system was realized with automated volume metering, microfluidic sample preparation, and integrated nano-biosensors.

Paper Details

Date Published: 18 February 2010
PDF: 11 pages
Proc. SPIE 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII, 75930S (18 February 2010); doi: 10.1117/12.844856
Show Author Affiliations
Jungkyu Kim, The Univ. of Utah (United States)
John Elsnab, The Univ. of Utah (United States)
Michael Johnson, The Univ. of Utah (United States)
Bruce K. Gale, The Univ. of Utah (United States)

Published in SPIE Proceedings Vol. 7593:
Microfluidics, BioMEMS, and Medical Microsystems VIII
Holger Becker; Wanjun Wang, Editor(s)

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