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Proceedings Paper

Quantification of fluorescent spots in time series of 3D confocal microscopy images of endoplasmic reticulum exit sites based on the HMAX transform
Author(s): Petr Matula; Fatima Verissimo; Stefan Wörz; Roland Eils; Rainer Pepperkok; Karl Rohr
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Paper Abstract

We present an approach for the quantification of fluorescent spots in time series of 3-D confocal microscopy images of endoplasmic reticulum exit sites of dividing cells. Fluorescent spots are detected based on extracted image regions of highest response using the HMAX transform and prior convolution of the 3-D images with a Gaussian kernel. The sensitivity of the involved parameters was studied and a quantitative evaluation using both 3-D synthetic and 3-D real data was performed. The approach was successfully applied to more than one thousand 3-D confocal microscopy images.

Paper Details

Date Published: 9 March 2010
PDF: 7 pages
Proc. SPIE 7626, Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging, 76261H (9 March 2010); doi: 10.1117/12.844441
Show Author Affiliations
Petr Matula, Univ. of Heidelberg (Germany)
BIOQUANT (Germany)
IPMB (Germany)
Fatima Verissimo, EMBL Heidelberg (Germany)
Stefan Wörz, Univ. of Heidelberg (Germany)
BIOQUANT (Germany)
IPMB (Germany)
Roland Eils, Univ. of Heidelberg (Germany)
BIOQUANT (Germany)
IPMB (Germany)
Rainer Pepperkok, EMBL Heidelberg (Germany)
Karl Rohr, Univ. of Heidelberg (Germany)
BIOQUANT (Germany)
IPMB (Germany)


Published in SPIE Proceedings Vol. 7626:
Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging
Robert C. Molthen; John B. Weaver, Editor(s)

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