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Proceedings Paper

Image formation of volume holographic microscopy using point spread functions
Author(s): Yuan Luo; Se Baek Oh; Shan Shan Kou; Justin Lee; Colin J. R. Sheppard; George Barbastathis
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Paper Abstract

We present a theoretical formulation to quantify the imaging properties of volume holographic microscopy (VHM). Volume holograms are formed by exposure of a photosensitive recording material to the interference of two mutually coherent optical fields. Recently, it has been shown that a volume holographic pupil has spatial and spectral sectioning capability for fluorescent samples. Here, we analyze the point spread function (PSF) to assess the imaging behavior of the VHM with a point source and detector. The coherent PSF of the VHM is derived, and the results are compared with those from conventional microscopy, and confocal microscopy with point and slit apertures. According to our analysis, the PSF of the VHM can be controlled in the lateral direction by adjusting the parameters of the VH. Compared with confocal microscopes, the performance of the VHM is comparable or even potentially better, and the VHM is also able to achieve real-time and three-dimensional (3D) imaging due to its multiplexing ability.

Paper Details

Date Published: 23 March 2010
PDF: 7 pages
Proc. SPIE 7622, Medical Imaging 2010: Physics of Medical Imaging, 76224E (23 March 2010); doi: 10.1117/12.844310
Show Author Affiliations
Yuan Luo, Massachusetts Institute of Technology (United States)
Se Baek Oh, Massachusetts Institute of Technology (United States)
Shan Shan Kou, National Univ. of Singapore (Singapore)
Graduate School for Integrative Sciences and Engineering (Singapore)
Justin Lee, Massachusetts Institute of Technology (United States)
Colin J. R. Sheppard, National Univ. of Singapore (Singapore)
Graduate School for Integrative Sciences and Engineering (Singapore)
George Barbastathis, Massachusetts Institute of Technology (United States)
Singapore-MIT Alliance for Research and Technology Ctr. (Singapore)


Published in SPIE Proceedings Vol. 7622:
Medical Imaging 2010: Physics of Medical Imaging
Ehsan Samei; Norbert J. Pelc, Editor(s)

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