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Proceedings Paper

3D motion analysis of keratin filaments in living cells
Author(s): Gerlind Herberich; Reinhard Windoffer; Rudolf Leube; Til Aach
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Paper Abstract

We present a novel and efficient approach for 3D motion estimation of keratin intermediate filaments in vitro. Keratin filaments are elastic cables forming a complex scaffolding within epithelial cells. To understand the mechanisms of filament formation and network organisation under physiological and pathological conditions, quantitative measurements of dynamic network alterations are essential. Therefore we acquired time-lapse series of 3D images using a confocal laser scanning microscope. Based on these image series, we show that a dense vector field can be computed such that the displacements from one frame to the next can be determined. Our method is based on a two-step registration process: First, a rigid pre-registration is applied in order to compensate for possible global cell movement. This step enables the subsequent nonrigid registration to capture only the sought local deformations of the filaments. As the transformation model of the deformable registration algorithm is based on Free Form Deformations, it is well suited for modeling filament network dynamics. The optimization is performed using efficient linear programming techniques such that the huge amount of image data of a time series can be efficiently processed. The evaluation of our results illustrates the potential of our approach.

Paper Details

Date Published: 12 March 2010
PDF: 9 pages
Proc. SPIE 7623, Medical Imaging 2010: Image Processing, 76232B (12 March 2010); doi: 10.1117/12.844148
Show Author Affiliations
Gerlind Herberich, RWTH Aachen Univ. (Germany)
Reinhard Windoffer, Univ. Hospital Aachen (Germany)
Rudolf Leube, Univ. Hospital Aachen (Germany)
Til Aach, RWTH Aachen Univ. (Germany)

Published in SPIE Proceedings Vol. 7623:
Medical Imaging 2010: Image Processing
Benoit M. Dawant; David R. Haynor, Editor(s)

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