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Proceedings Paper

Optical-resolution photoacoustic microscopy of amyloid-β deposits in vivo
Author(s): Song Hu; Ping Yan; Konstantin Maslov; Jin-Moo Lee; Lihong V. Wang
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Paper Abstract

Advances in high-resolution imaging have permitted microscopic observations within the brains of living animals. Applied to Alzheimer's disease (AD) mouse models, multiphoton microscopy has opened a new window to study the real-time appearance and growth of amyloid plaques. Here, we report an alternative technology-optical-resolution photoacoustic microscopy (OR-PAM)-for in vivo imaging of amyloid plaques in a transgenic AD mouse model. In vivo validation using multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window. In vivo transcranial OR-PAM imaging of amyloid plaques is highly likely once the imaging parameters are optimized.

Paper Details

Date Published: 23 February 2010
PDF: 4 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75643D (23 February 2010); doi: 10.1117/12.843919
Show Author Affiliations
Song Hu, Washington Univ. in St. Louis (United States)
Ping Yan, Washington Univ. in St. Louis (United States)
Konstantin Maslov, Washington Univ. in St. Louis (United States)
Jin-Moo Lee, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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