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Proceedings Paper

Immobilization of human papillomavirus DNA probe for surface plasmon resonance imaging
Author(s): Xinyuan Chong; Yanhong Ji; Suihua Ma; Le Liu; Zhiyi Liu; Yao Li; Yonghong He; Jihua Guo
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Paper Abstract

Human papillomavirus (HPV) is a kind of double-stranded DNA virus whose subspecies have diversity. Near 40 kinds of subspecies can invade reproductive organ and cause some high risk disease, such as cervical carcinoma. In order to detect the type of the subspecies of the HPV DNA, we used the parallel scan spectral surface plasmon resonance (SPR) imaging technique, which is a novel type of two- dimensional bio-sensing method based on surface plasmon resonance and is proposed in our previous work, to study the immobilization of the HPV DNA probes on the gold film. In the experiment, four kinds of the subspecies of the HPV DNA (HPV16, HPV18, HPV31, HPV58) probes are fixed on one gold film, and incubate in the constant temperature condition to get a HPV DNA probe microarray. We use the parallel scan spectral SPR imaging system to detect the reflective indices of the HPV DNA subspecies probes. The benefits of this new approach are high sensitive, label-free, strong specificity and high through-put.

Paper Details

Date Published: 29 October 2009
PDF: 6 pages
Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75190O (29 October 2009); doi: 10.1117/12.843886
Show Author Affiliations
Xinyuan Chong, Tsinghua Univ. (China)
Yanhong Ji, South China Normal Univ. (China)
Suihua Ma, Tsinghua Univ. (China)
Le Liu, Tsinghua Univ. (China)
Zhiyi Liu, Tsinghua Univ. (China)
Yao Li, Tsinghua Univ. (China)
Yonghong He, Tsinghua Univ. (China)
Jihua Guo, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 7519:
Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Pengcheng Li; Ling Fu, Editor(s)

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