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Proceedings Paper

Simultaneous observation of ultrafast ligand dissociation and docking-site trapping in heme proteins using upconversion infrared spectroscopy
Author(s): Patrick Nuernberger; Kevin F. Lee; Adeline Bonvalet; Antigoni Alexandrou; Marten H. Vos; Manuel Joffre
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Paper Abstract

We report on ultrafast visible pump/mid-infrared probe spectroscopy of the carboxy form of heme proteins by employing the recently developed chirped-pulse upconversion technique, which allows both high resolution and sensitivity over an extremely broad spectral range. Commonly, the bleach signal due to ligand dissociation and the incipient docking-site absorption signal, being about 200 cm-1 apart and differing by more than an order of magnitude in absorptivity, are studied in separate experiments. We here monitor them simultaneously, allowing a direct observation and a concurrent analysis of the initial processes after photoinduced ligand dissociation, for instance, the formation of hot vibrational bands.

Paper Details

Date Published: 25 February 2010
PDF: 8 pages
Proc. SPIE 7560, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry, 75600F (25 February 2010); doi: 10.1117/12.843672
Show Author Affiliations
Patrick Nuernberger, Lab. d'Optique et Biosciences, CNRS, Ecole Polytechnique (France)
Institut National de la Santé et de la Recherche Médicale (France)
Kevin F. Lee, Lab. d'Optique et Biosciences, CNRS, Ecole Polytechnique (France)
Institut National de la Santé et de la Recherche Médicale (France)
Adeline Bonvalet, Lab. d'Optique et Biosciences, CNRS, Ecole Polytechnique (France)
Institut National de la Santé et de la Recherche Médicale (France)
Antigoni Alexandrou, Lab. d'Optique et Biosciences, CNRS, Ecole Polytechnique (France)
Institut National de la Santé et de la Recherche Médicale (France)
Marten H. Vos, Lab. d'Optique et Biosciences, CNRS, Ecole Polytechnique (France)
Institut National de la Santé et de la Recherche Médicale (France)
Manuel Joffre, Lab. d'Optique et Biosciences, CNRS, Ecole Polytechnique (France)
Institut National de la Santé et de la Recherche Médicale (France)


Published in SPIE Proceedings Vol. 7560:
Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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