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Proceedings Paper

Measurement of glucose concentration in interstitial fluid by surface plasmon resonance with D-galactose/D-glucose binding protein
Author(s): D. C. Li; J. X. Zhang; P. Wu; F. X. Huang; B. Song; K. X. Xu
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Paper Abstract

A novel minimally invasive way to measure blood glucose concentration is proposed by combining interstitial fluid transdermal extraction and surface plasma resonance (SPR) detecting. 55K Hz low-frequency ultrasound pulse is applied for less than 30 seconds to enhance the skin permeability and then interstitial fluid is extracted out of skin by vacuum. The mathematical model to express the correlation between interstitial fluid glucose and blood glucose is also developed by considering the changes of the skin conductivity. The glucose concentration in the interstitial fluid is determined using an optical SPR biological sensor that measures the refractive index. A protein-glucose binding technology using Dgalactose/ D-glucose Binding Protein for specific absorption of glucose is employed to increase SPR measurement precision. By immobilizing GGBP onto the surface of the SPR sensor, the experimental result indicates the detecting resolution of glucose rises to 1mg/L, the system succeeds in distinguishing glucose from other components in mixture. The feasibility of this method is validated for clinical application with the requirements of bloodless, painless, continuous glucose monitoring and a prototype microfluidic diabetes-monitoring device is under development.

Paper Details

Date Published: 28 October 2009
PDF: 7 pages
Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75190V (28 October 2009); doi: 10.1117/12.843610
Show Author Affiliations
D. C. Li, Tianjin Univ. (China)
J. X. Zhang, Tianjin Univ. (China)
P. Wu, Tianjin Univ. (China)
F. X. Huang, Tianjin Univ. (China)
B. Song, Tianjin Univ. (China)
K. X. Xu, Tianjin Univ. (China)

Published in SPIE Proceedings Vol. 7519:
Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Pengcheng Li; Ling Fu, Editor(s)

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