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Proceedings Paper

Discrimination of collagen in normal and pathological dermis through polarization second harmonic generation
Author(s): Ping-Jung Su; Wei-Liang Chen; Jin-Bon Hong; Tsung-Hsien Li; Ruei-Jr Wu; Chen-Kuan Chou; Sung-Jan Lin; Chen-Yuan Dong
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Paper Abstract

We used polarization-resolved, second harmonic generation (P-SHG) microscopy at single pixel resolution for medical diagnosis of pathological skin dermis, and found that P-SHG can be used to distinguish normal and dermal pathological conditions of keloid, morphea, and dermal elastolysis. We find that the histograms of the d33/d31 ratio for the pathological skins to contain two peak values and to be wider than that of the normal case, suggesting that the pathological dermal collagen fibers tend to be more structurally heterogeneous. Our work demonstrates that pixel-resolved, second-order susceptibility microscopy is effective for detecting heterogeneity in spatial distribution of collagen fibers.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692A (26 February 2010); doi: 10.1117/12.843370
Show Author Affiliations
Ping-Jung Su, National Taiwan Univ. (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Jin-Bon Hong, National Taiwan Univ. Hospital (Taiwan)
Tsung-Hsien Li, National Taiwan Univ. (Taiwan)
Ruei-Jr Wu, National Taiwan Univ. (Taiwan)
Chen-Kuan Chou, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. (Taiwan)
National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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