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Proceedings Paper

Visualizing photodynamic therapy response with time-lapse OCT in an in vitro model of metastatic ovarian cancer
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Paper Abstract

Ovarian epithelial cancer has a high morbidity due to its propensity to metastasize onto surfaces in the abdomen. In order to effectively treat these metastatic lesions with photodynamic therapy (PDT), it is critical to understand the detailed dynamics of the PDT response. 3D in vitro models of ovarian cancer are a promising system for studying the response to PDT of these lesions, as they replicate the size, appearance, and characteristics of metastatic disease observed in the clinic. An ideal approach capable of non-purturbative, 3D imaging of this model is optical coherence tomography (OCT). An ultrahigh resolution time-lapse OCT (TL-OCT) system was used to visualize the photodynamic therapeutic response in the hours and days following treatment. Tumor nodules were observed to experience rapid cell death within the first 24 hours post-treatment using benzophorphyrin derivative monoacid A (BPD), characterized by structural breakdown of the model nodules. Highly scattering bodies were observed with OCT contrast to form at the periphery of the tumor nodules. These highly scattering moieties were identified as apoptotic bodies, indicating that OCT is capable of tracking the PDT-induced apoptosis in real-time without the need for labels.

Paper Details

Date Published: 5 March 2010
PDF: 5 pages
Proc. SPIE 7551, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX, 75510J (5 March 2010); doi: 10.1117/12.843271
Show Author Affiliations
Conor L. Evans, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)
Imran Rizvi, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)
Dartmouth College (United States)
Jonathan Celli, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)
Adnan Abu-Yousif, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)
Johannes de Boer, Vrije Univ. Amsterdam (Netherlands)
Tayyaba Hasan, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)


Published in SPIE Proceedings Vol. 7551:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX
David H. Kessel, Editor(s)

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