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Proceedings Paper

Mutant and wild type cell chemotaxis in 3D and 4D with ultrahigh-resolution optical coherence tomography
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Paper Abstract

Conventionally, cell chemotaxis is studied on two-dimensional (2D) transparent surfaces due to limitations in optical and image data-collection techniques. However, substrates which more closely mimic the natural environment of cells are often opaque or three-dimensional (3D). The non-invasive label-free imaging technique of frequency domain optical coherence tomography (OCT) has high axial and transverse resolution of >4μm, comparatively high penetration depth and the ability to acquire volumes in a few seconds, therefore offering the potential to visualize moving cells in 3D (2D+time) and 4D (3D+time). Cell migration is demonstrated in 3D on opaque surfaces, and in 4D within an agarose gel. The speed and directionality of wild type (Ax2) cell movement is seen to be comparable on agar and nitrocellulose filter substrates. Differences can be clearly seen in the character of cell movement between Ax2, myosin knockout (mhcA-) and PLC null ~10μm Dictyostelium discoideum cells in 4D using ultrahigh resolution OCT. OCT is therefore shown to be a useful technique for the study of cell migration.

Paper Details

Date Published: 23 February 2010
PDF: 6 pages
Proc. SPIE 7554, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV, 75540N (23 February 2010); doi: 10.1117/12.843244
Show Author Affiliations
Sara M. Rey, Cardiff Univ. (United Kingdom)
Boris Považay, Medical Univ. of Vienna (Austria)
Cardiff Univ. (United Kingdom)
Bernd Hofer, Medical Univ. of Vienna (Austria)
Cardiff Univ. (United Kingdom)
Angelika Unterhuber, Medical Univ. of Vienna (Austria)
Cardiff Univ. (United Kingdom)
Adrian Harwood, Cardiff Univ. (United Kingdom)
Wolfgang Drexler, Medical Univ. of Vienna (Austria)
Cardiff Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 7554:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

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