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Proceedings Paper

Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy
Author(s): Wen Lo; Chih-Ting Hsu; Tsung-Rong Kuo; Chung-Long Wu; Shu-Jen Chiang; Sung-Jan Lin; Shean-Jen Chen; Chia-Chun Chen; Chen-Yuan Dong
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Paper Abstract

Zinc oxide nanoparticles (ZnO NPs) are commonly used in sunscreens to reduce the risk of skin cancer by blocking ultraviolet radiation. ZnO NPs absorption through the transdermal route may not cause high health risk as inhalation or ingestion. However, in practical usage of sunscreens and cosmetics, ZnO NPs are topically applied to a large area of skin with long periods hence the potential absorption amount of ZnO NPs is still need to be concerned. Therefore, if the ZnO NPs are able the pass the barrier of normal skin, the pathways of transdermal delivery and the factors of enhancements become important issues. In this work, multiphoton microscopy provides us a non-invasive visualization of ZnO NPs in skin. Moreover, we quantitatively analyzed the enhancement of oleic acid and ethanol. Due to the fact that photoluminance of ZnO NPs spectrally overlaps autofluorence from skin stratum corneum (SC) and high turbidity of both ZnO NPs and SC, it is difficult to resolve the distribution of ZnO NPs in skin by using fluorescence microscopy. In this work, the second harmonic generation (SHG) signals from ZnO NPs which double the frequency of excitation source to characterize the delivery pathways and penetration depth in skin. Moreover, we quantitatively compare the ZnO NPs delivery efficiency in normal skin and in skins with three chemically enhancing conditions: ethanol, oleic acid and the combination of ethanol and oleic acid.

Paper Details

Date Published: 2 March 2010
PDF: 4 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75480P (2 March 2010); doi: 10.1117/12.843187
Show Author Affiliations
Wen Lo, National Taiwan Univ. (Taiwan)
National Cheng Kung Univ. (Taiwan)
Chih-Ting Hsu, National Taiwan Univ. (Taiwan)
Tsung-Rong Kuo, National Taiwan Normal Univ. (Taiwan)
Chung-Long Wu, National Taiwan Normal Univ. (Taiwan)
Shu-Jen Chiang, Academia Sinica (Taiwan)
Sung-Jan Lin, National Taiwan Univ. (Taiwan)
National Taiwan Univ. Hospital (Taiwan)
Shean-Jen Chen, National Cheng Kung Univ. (Taiwan)
Chia-Chun Chen, National Taiwan Normal Univ. (Taiwan)
Academia Sinica (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong; Nikiforos Kollias; Henry Hirschberg; Kenton W. Gregory; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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