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Proceedings Paper

Characterization of corneal edema by forward and backward second harmonic generation microscopy
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Paper Abstract

We used second harmonic generation (SHG) microscopy to image and quantify the structural changes of bovine corneal edema. Forward SHG (FWSHG) and backward SHG (BWSHG) signals were simultaneously collected from normal and edematous bovine corneas to reveal their morphological differences. In SHG imaging, edematous corneas can be characterized by uneven expansion in the lamellar interspacing and increased lamellar thickness in posterior stroma (depth > 200 μm), while the anterior stroma composed of interwoven collagen architecture remained unaffected. Our work demonstrate the capability of SHG imaging in providing morphological information for the investigation of corneal edema biophysics and its potential application in the in vivo evaluation of advancing corneal edema.

Paper Details

Date Published: 2 March 2010
PDF: 4 pages
Proc. SPIE 7550, Ophthalmic Technologies XX, 75501M (2 March 2010); doi: 10.1117/12.843181
Show Author Affiliations
Chiu-Mei Hsueh, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Vladimir A. Hovhannisyan, National Taiwan Univ. (Taiwan)
Hsin-Yuan Tan, Institute of Medical Engineering, National Taiwan Univ. (Taiwan)
Chang Gung Memorial Hospital, Chang Gung Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 7550:
Ophthalmic Technologies XX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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