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Proceedings Paper

Differentiation of normal and cancerous lung tissues by multiphoton imaging
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Paper Abstract

In this work, we utilized multiphoton microscopy for the label-free diagnosis of non-cancerous, lung adenocarcinoma (LAC), and lung squamous cell carcinoma (SCC) tissues from human. Our results show that the combination of second harmonic generation (SHG) and multiphoton excited autofluorescence (MAF) signals may be used to acquire morphological and quantitative information in discriminating cancerous from non-cancerous lung tissues. Specifically, non-cancerous lung tissues are largely fibrotic in structure while cancerous specimens are composed primarily of tumor masses. Quantitative ratiometric analysis using MAF to SHG index (MAFSI or SAAID) shows that the average MAFSI for noncancerous and LAC lung tissue pairs are 0.55 ±0.23 and 0.87±0.15 respectively. In comparison, the MAFSIs for the noncancerous and SCC tissue pairs are 0.50±0.12 and 0.72±0.13 respectively. Intrinsic fluorescence ratio (FAD/NADH) of SCC and non-cancerous tissues are 0.40±0.05 and 0.53±0.05 respectively, the redox ratio of SCC diminishes significantly, indicating that increased cellular metabolic activity. Our study shows that nonlinear optical microscopy can assist in differentiating and diagnosing pulmonary cancer from non-cancerous tissues. With additional development, multiphoton microscopy may be used for the clinical diagnosis of lung cancers.

Paper Details

Date Published: 11 February 2010
PDF: 6 pages
Proc. SPIE 7561, Optical Biopsy VII, 756112 (11 February 2010); doi: 10.1117/12.843173
Show Author Affiliations
Chun-Chin Wang, National Taiwan Univ. (Taiwan)
Feng-Chieh Li, National Taiwan Univ. (Taiwan)
Ruei-Jr Wu, National Taiwan Univ. (Taiwan)
Vladimir A. Hovhannisyan, National Taiwan Univ. (Taiwan)
Wei-Chou Lin, National Taiwan Univ. Hospital (Taiwan)
Sung-Jan Lin, National Taiwan Univ. (Taiwan)
National Taiwan Univ. Hospital (Taiwan)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 7561:
Optical Biopsy VII
Robert R. Alfano, Editor(s)

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