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Proceedings Paper

Assessment of fibrotic liver disease with multimodal nonlinear optical microscopy
Author(s): Fake Lu; Wei Zheng; Dean C. S. Tai; Jian Lin; Hanry Yu; Zhiwei Huang
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Paper Abstract

Liver fibrosis is the excessive accumulation of extracellular matrix proteins such as collagens, which may result in cirrhosis, liver failure, and portal hypertension. In this study, we apply a multimodal nonlinear optical microscopy platform developed to investigate the fibrotic liver diseases in rat models established by performing bile duct ligation (BDL) surgery. The three nonlinear microscopy imaging modalities are implemented on the same sectioned tissues of diseased model sequentially: i.e., second harmonic generation (SHG) imaging quantifies the contents of the collagens, the two-photon excitation fluorescence (TPEF) imaging reveals the morphology of hepatic cells, while coherent anti-Stokes Raman scattering (CARS) imaging maps the distributions of fats or lipids quantitatively across the tissue. Our imaging results show that during the development of liver fibrosis (collagens) in BDL model, fatty liver disease also occurs. The aggregated concentrations of collagen and fat constituents in liver fibrosis model show a certain correlationship between each other.

Paper Details

Date Published: 26 February 2010
PDF: 4 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75691W (26 February 2010); doi: 10.1117/12.843127
Show Author Affiliations
Fake Lu, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Dean C. S. Tai, Institute of Bioengineering and Nanotechnology (Singapore)
Jian Lin, National Univ. of Singapore (Singapore)
Hanry Yu, Institute of Bioengineering and Nanotechnology (Singapore)
National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)


Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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