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Proceedings Paper

Polarized fluorescence study in human cervical tissue: change in autofluorescence through different excitation wavelengths
Author(s): Rajbeer Singh; Krishna Kumar Singh Tomar; Meghdoot Majumdar; Prashant Shukla; Asima Pradhan; Rekha Gupta; Sonal Jain; Chayanika Pantola; Asha Agarwal; Kiran Pandey
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Paper Abstract

This study aims towards applying the intrinsic fluorescence technique, extracted from polarized fluorescence, to detect subtle biochemical changes occurring during the progression of cancer from human cervical tissue samples. The efficacy of this technique, earlier validated through tissue phantoms, is tested in human cervical tissues by comparing the biochemical changes for diagnostic purpose at different wavelengths. It is pertinent to note that the co and crosspolarized fluorescence do not display the high sensitivity obtained through extracted intrinsic fluorescence. We observed that sensitivity and specificity of intrinsic fluorescence technique is high at 325 and 370nm for Collagen and NADH respectively in comparison to 350nm excitation wavelength. It may be concluded that decoupled information at 325 and 370nm wavelengths for collagen and NADH respectively, through intrinsic fluorescence provides better diagnostic parameter for early detection of cervical dysplasia. This information can provide a guiding path for designing a probe for clinical purpose.

Paper Details

Date Published: 11 February 2010
PDF: 8 pages
Proc. SPIE 7561, Optical Biopsy VII, 75610J (11 February 2010); doi: 10.1117/12.843118
Show Author Affiliations
Rajbeer Singh, Indian Institute of Technology Kanpur (India)
Krishna Kumar Singh Tomar, Indian Institute of Technology Kanpur (India)
Meghdoot Majumdar, Indian Institute of Technology Kanpur (India)
Prashant Shukla, Indian Institute of Technology Kanpur (India)
Asima Pradhan, Indian Institute of Technology Kanpur (India)
Rekha Gupta, Ganesh Shanker Vidyarthi Memorial Medical College (India)
Sonal Jain, Ganesh Shanker Vidyarthi Memorial Medical College (India)
Chayanika Pantola, Ganesh Shanker Vidyarthi Memorial Medical College (India)
Asha Agarwal, Ganesh Shanker Vidyarthi Memorial Medical College (India)
Kiran Pandey, Ganesh Shanker Vidyarthi Memorial Medical College (India)


Published in SPIE Proceedings Vol. 7561:
Optical Biopsy VII
Robert R. Alfano, Editor(s)

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