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Proceedings Paper

Dynamic phase imaging of host cells attacked by Vibrio vulnificus using quantitative phase microscopy
Author(s): Seungrag Lee; Wenzhong Yang; Ji Yong Lee; Mi Hye Cha; Young Ran Kim; Dug Young Kim
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Paper Abstract

We present the real time quantitative analysis of Vibrio vulnificus-infected host cells using high stability quantitative phase microscopy (HSQPM). It provides the ability to retrieve the phase or optical path length distribution over the cell from a single interferogram image, which has been measured with nanometer path length sensitivity for long periods of time. We have applied HSQPM to study dynamic cell morphologic changes and to quantify noninvasively cell volumes of rat basophilic leukemia RBL-2H3 cells infected with pathogenic bacteria V. vulnificus strains, wild type (MO6-24/O) and RTX toxin mutant (CMM770). During the process of V. vulnificus wild type infection to RBL-2H3 cells, the dynamic changes of quantitative phase images, cell volumes and areas were observed in real time using HSQPM. In contrast, the dramatic changes were not detected in RBL-2H3 cells infected with RTX toxin mutant. The results showed the good correlation between HSQPM analysis and biochemical assays such as lactate dehydrogenase (LDH) assay and β-hexosaminidase release assay. We suggest that HSQPM is useful real time quantitative method to study the dynamic process of host cells infected with pathogen in a noninvasive manner.

Paper Details

Date Published: 25 February 2010
PDF: 9 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756808 (25 February 2010); doi: 10.1117/12.843057
Show Author Affiliations
Seungrag Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Wenzhong Yang, Gwangju Institute of Science and Technology (Korea, Republic of)
Ji Yong Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Mi Hye Cha, Dongshin Univ. (Korea, Republic of)
Young Ran Kim, Dongshin Univ. (Korea, Republic of)
Dug Young Kim, Gwangju Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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