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Proceedings Paper

Spectral ophthalmoscopy based on supercontinuum
Author(s): Yueh-Hung Cheng; Jiun-Yann Yu; Han-Hsuan Wu; Bo-Jyun Huang; Shi-Wei Chu
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Paper Abstract

Confocal scanning laser ophthalmoscope (CSLO) has been established to be an important diagnostic tool for retinopathies like age-related macular degeneration, glaucoma and diabetes. Compared to a confocal laser scanning microscope, CSLO is also capable of providing optical sectioning on retina with the aid of a pinhole, but the microscope objective is replaced by the optics of eye. Since optical spectrum is the fingerprint of local chemical composition, it is attractive to incorporate spectral acquisition into CSLO. However, due to the limitation of laser bandwidth and chromatic/geometric aberration, the scanning systems in current CSLO are not compatible with spectral imaging. Here we demonstrate a spectral CSLO by combining a diffraction-limited broadband scanning system and a supercontinuum laser source. Both optical sectioning capability and sub-cellular resolution are demonstrated on zebrafish's retina. To our knowledge, it is also the first time that CSLO is applied onto the study of fish vision. The versatile spectral CSLO system will be useful to retinopathy diagnosis and neuroscience research.

Paper Details

Date Published: 24 February 2010
PDF: 5 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680I (24 February 2010); doi: 10.1117/12.843020
Show Author Affiliations
Yueh-Hung Cheng, National Taiwan Univ. (Taiwan)
Jiun-Yann Yu, National Taiwan Univ. (Taiwan)
Han-Hsuan Wu, National Taiwan Univ. (Taiwan)
Bo-Jyun Huang, National Taiwan Univ. (Taiwan)
Shi-Wei Chu, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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